Sport Sciences and Health Research

Sport Sciences and Health Research

The effect of 8 weeks of quercetin supplementation and intermittent exercise on gene expression of Muc5Ac, Muc4 and polyphosphate in rats with colon cancer

Document Type : Research Paper

Authors
Shahin Heydari 1
Khadijeh Irandoust 1
Morteza Taheri 2
1 Department of Sport Sciences, Faculty of Social Sciences, Imam Khomeini International University, Qazvin, Iran.
2 Department of Behavioural and Cognitive Sciences in Sport, Faculty of Sport Science and Health, University of Tehran, Tehran, Iran.
10.22059/sshr.2023.357150.1081
Abstract
Background: Expression of mucosal levels would affect the function of internal organs of the body and the digestive system, such as by creating a blockage for the progression of cancerous tumors and the failure of the target tissue, especially the large intestine.
Aim: The purpose of this sudy was to investigate the effect of 8 weeks of quercetin supplementation and intermittent exercise on protein levels of intestinal Muc5Ac, Muc4 and polyphosphate in rats with colon cancer.
Material and Methods: Tewenty-four rats were randomly assigned into four groups including quercetin (n=6), exercise (n=6), quercetin + exercise (n=6) and control group (n=6). Colon cancer induction was provided with the use of 1,2-dimethylhydrazine for 8 weeks and daily quercetin supplementation of 50 mg/kg body weight of mice by Gavagene method. Exercise protocol was performed 5 sessions per week with intensity of 60-70%, maximum speed of 23 m/min with 2-min rest in 8 weeks. ANOVA was used to analyze data. The level of significance was set at P<0.05.
Results: It was suggested that there was a significant difference in protein levels of intestinal Muc5Ac, Muc4 and polyphosphate in all groups (P<0.05). Furthermore, it was also indicated that Muc5Ac levels was significantly higher in the quercetin+ exercise group other than pther groups (P<0.05).
Conclusions: It was concluded that intermittent exercise and quercetin supplementation would increase the levels of Muc5Ac and Muc4 proteins in the large intestine of mice with colon cancer.  
Keywords
intermittent exercise
Muc4
Muc5Ac
polyphosphate
quercetin supplement

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Sport Sciences and Health Research
Volume 15, Issue 1
January 2023
Pages 13-22