University of Tehran Sport Sciences and Health Research 2981-0205 17 2 2025 07 01 Aerobic Exercise Prevents Memory Deficits Induced by Electromagnetic Radiation by Altering Cholinergic Biomarkers and Amyloid Beta Levels 12 29 106115 10.22059/sshr.2025.381752.1161 EN Monireh Esmaeili Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran Parisa Pournemati Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran Fatemeh Shabkhiz Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran Mohammadreza Kordi Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran Journal Article 2024 11 16 <strong>Background</strong>: As technology advances, concerns regarding neurobehavioral disorders, particularly memory deficits caused by electromagnetic radiation (EMR) from wireless devices such as Wi-Fi routers have increased dramatically.<br /><strong>Aim: </strong>This study aimed to investigate the impact of aerobic exercise on cholinergic biomarkers and amyloid beta (Aβ) levels in the hippocampus of male Wistar rats exposed to 2450MHz EMR emitted by Wi-Fi routers.<br /><strong>Materials and Methods: </strong>Eighteen rats were randomly divided into three groups: 'control', 'wave', and 'wave+aerobic exercise'. During four weeks, the 'wave' groups were exposed to 2450MHz EMR from Wi-Fi routers for four hours daily, seven days a week the 'wave+aerobic exercise' group also participated in a running program five days a week in addition to EMR exposure. Cognitive behavioral changes resulting from the interventions were evaluated using the Morris Water Maze (MWM) test.<br /><strong>Results: </strong>Exposure to EMR significantly decreased acetylcholine and acetylcholinesterase levels, and increased Aβ levels in the hippocampus (P<0.05). The MWM test also revealed a significant impairment in spatial memory among the exposed rats compared to the 'control' group. However, the 'wave+aerobic exercise' group exhibited a significant increase in acetylcholine and acetylcholinesterase levels, a significant reduction in Aβ levels, and demonstrated considerably better performance in the MWM test compared to the 'wave' group (P<0.05).<br /><strong>Conclusion: </strong>The findings of this study demonstrate the beneficial effects of aerobic exercise in preventing pathophysiological alterations and cognitive impairments caused by electromagnetic radiation. https://sshr.ut.ac.ir/article_106115_2fb4c882de8c7c3b910d4ef29b5b4e3e.pdf